The PCR laboratory is a shared laboratory within the department of Plant Pathology. It currently occupies two labs (Rm 231 and 202) and is supervised by Melanie Lewis Ivey. In addition to bench space the labs provide access to several pieces of equipment essential to PCR. Support for the PCR lab comes from faculty in the departments of Plant Pathology, Entomology, Food, Agricultural and Biological Engineering and from OARDC matching grants.

 
Equipment
Lab and Equipment Instructions

Thermocyclers (Rm 231)
- 4 MJR PTC 100 thermocyclers
- 1 MJR PTC 200 thermocycler with a gradient alpha block
- 1 MJR (BioRad) Tetrad 2 thermocycler with 96 well blocks
- 1 dual alpha block (48 wells each)

Electrophoresis Equipment (Rm 202 and 231)
- gel boxes of various sizes (horizontal and vertical)
- power boxes
- staining and destaining boxes
- shaking platform

Spectrophotometres (Rm 231)
- 1 Shimadzu BioSpec
- 2 50-100 uL quartz cuvettes
- 1 1ml quartz cuvette
- 1 Nanodrop ND1000


Imaging System (Rm 202)

- Kodak digital camera
- Kodak 2D Imaging Software
- iMac computer
- Photoshop software
- Adobe Illustrator software

Centrifuge (Rm 202)
- Beckman refrigerated table top centrifuge
- 96 well plate rotor
- rotors for 250 and 50 ml tubes
- inserts to accomondate 15 ml tubes

Microcentrifuges (Rm 202 and 231)
- 2 at room temperature
- 1 at 4 C

PCR Lab Rules and Regulations
Thermocyclers
Imaging System
Imaging Computer
Table Top Centrifuge
Spectrophotometre (BioSpec)
Spectrophotometre (Nanodrop)
pH Metre

 
Promega Freezer and Cabinet

The Promega Express freezers and cabinet are located in Selby Hall. To obtain an access key for the freezer please contact Melanie Lewis Ivey (ivey.14@osu.edu or ext. 2704) for instructions. The freezers and cabinet are open from 8-12 and 1-4 pm, Monday to Friday.

Requests for product additions can be made on-line at the Promega Express site (click icon below)

To view a list of items stocked in the freezers and cabinet log onto www.promega.com and click on the Promega Express icon.