Automated Image Collection and Analysis
GFP Robotics
Since 2001, we have been developing a robotics system for automated automated image collection of GFP expression in tissues growing in Petri dishes.
This robotics system allows collection of images from multiple samples, in Peb tri dishes, which are mounted on a 2 dimensional robotics platform. The platform is computer-controlled, and tissues are precisely placed under the objective of a MZFLIII fluorescent dissecting microscope at specific times. Images are collected using a Spot camera, controlled via custom software.
Image collection, followed by image analysis, allows the generation of large amount of data quantifying gene expression. Assembly of the images yields digital time-lapse animations of tissue growth and gene expression. See our protocol in the Journal of Visualized Experiments.
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GFP detection robotics system with 2-dimensional robotics table, Leica MZFLIII micrscope and Spot camera, all under computer control. |
| Rapid animation of robotics platform movement. Petri dish contains lima bean cotyledons, used for soybean promoter analysis. |
Publications resulting from this image collection
and analysis system:
Hernandez-Garcia CM, Chiera JM, Finer JJ (2010) Robotics and dynamic image analysis for studies of gene expression in plant tissues. Journal of Visualized Experiments http://www.jove.com/index/Details.stp?ID=1733
Finer JJ (in press) In (eds: CN Stewart, A Touraev, V Citovsky, T Tzfira) Plant Transformation Technologies, “Visualizing transgene expression” Wiley Publishing Co. Hoboken, NJ
Dhillon T, Chiera JM, Lindbo JA, Finer JJ (2009) Quantitative evaluation of six different viral suppressors of silencing using image analysis of transient GFP expression. Plant Cell Reports 28:639-647
Wu C, Chiera JM, Ling PP, Finer JJ (2008) Isoxaflutole treatment leads to reversible tissue bleaching and allows for more effective detection of GFP in transgenic soybean tissues. In Vitro Cellular and Developmental Biology – Plant 6:540-547
Chiera JM, Lindbo JA, Finer JJ (2008) Quantification and extension of transient GFP expression by the co-introduction of a suppressor of silencing. Transgenic Research 17:1143-1154
Finer JE, Finer JJ (2007) A simple method for reducing moisture condensation on Petri dish lids. Plant Cell Tissue and Organ Culture 91:299-304
Chiera JM, Bouchard RA, Dorsey SL, Park EH, Buenrostro-Nava MT, Ling PP, Finer JJ (2007) Isolation of two highly active soybean (Glycine max (L.) Merr.) promoters and their characterization using a new automated image collection and analysis system. Plant Cell Reports. 26:1501-1509
Finer JJ (2006) Tracking of transgene expression in soybean using robotics and GFP. In Proceedings of the International Symposium of Plant Biotechnology, "Prospects of Plant Biotechnology in Bio-industry", Institute of Biotechnology, Yeungnam University, Korea, pp 17-22 - pdf
Buenrostro-Nava MT, Ling PP, Finer JJ (2006) Comparative analysis of 35S and Lectin promoters in transgenic soybean tissue using an automated image acquisition system and image analysis. Plant Cell Reports 25:290-296
Finer JJ, Beck SL, Buenrostro-Nava MT, Chi YT, Ling PP (2006) In (Eds. S Dutta Gupta, Y Ibaraki) Plant Tissue Culture Engineering, 31-46 “Monitoring Gene Expression in Plant Tissues; Using green fluorescent protein with automated image collection and analysis” Springer, Dordrecht
Buenrostro-Nava MT, Ling PP, Finer JJ (2005) Development of an automated image acquisition system for monitoring gene expression and tissue growth. Transactions of the Amer Soc for Agric Eng 42:841-847- pdf
Buenrostro-Nava MT, Ling PP, Finer JJ (2003) In (Ed. I Vasil) Plant Biotechnology 2002 and Beyond. 293-295 “Development of an automated image collection system for generating time-lapse animations of plant tissue growth and green fluorescent protein gene expression.” Kluwer Acad Pub, Dordrecht
