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Hernandez-Garcia, Carlos M.

Charles E. Thorne Memorial Fellow

PhD Candidate

Dep. of Horticulture and Crop Science

1680 Madison Ave.

Williams Hall

Wooster, OH 44691

Ph# 330.263.3979

hernandez-garcia.1@buckeyemail.osu.edu

 

Making the cover and the back of the MIDSCI's 2012 Quick Shope Guide with pictures of our exceptional strong ubiquitin promoter in soybean!


View Carlos M. Hernandez-Garcia's profile on LinkedIn

I am currently a 5-year PhD student in the Finer laboratory working on isolation and characterization of soybean promoters. Specifically, my ongoing research involves extensive functional characterization of GmERF (Glycine max Ethylene Response Factors) genes and their promoters in soybean. This gene family mediates different stress-induced responses in plants and its study will provide novel insights into regulation of gene expression. This will also enable to discover novel soybean inducible promoters with potential application in basic research or the development of improved transgenic crops.

 

Educational Background

PhD Student.- The Ohio State University/OARDC, Department of Horticulture and Crop Science (2007-present).

M.Sc.- Postgraduate College in Agricultural Sciences (Mexico), Plant Genetics (2003-2006). Graduate Student at the Applied Biotechnology Center of the International Maize and Wheat Improvement Center (CIMMYT).

B.S.- University of Chapingo (Mexico), Major in Tropical Agriculture (1998-2002).

 

Publications

Hernandez-Garcia CM, Bouchard RA, Rushton PJ, Chen X, Timko MP, Finer JJ (2010) High level transgenic expression of soybean (Glycine max) GmERF and Gmubi gene promoters isolated by a novel promoter analysis pipeline. BMC Plant Biology 10:237

Hernandez-Garcia CM, Chiera JM, Finer JJ (2010) Robotics and dynamic image analysis for studies of gene expression in plant tissues. Journal of Visualized Experiments 39 http://www.jove.com/index/details.stp?id=1733

Hernandez-Garcia CM, Martinelli AP, Bouchard RA, Finer JJ (2009) A soybean (Glycine max) polyubiquitin promoter gives strong constitutive expression in transgenic soybean. Plant Cell Rep 28:837-849 (pdf)

Hernandez-Garcia CM, Lopez-Peralta MC, Buenrostro-Nava MT, Pellegrineschi A, Cardenas-Soriano E (2007) Regeneration of subtropical white maize via somatic embryogenesis. Agrociencia 41:743-753 (pdf).

 

Abstracts and Presentations

Hernandez-Garcia CM, Nemes CA, De la Torre C, Semsang N, Jones ML, Finer JJ. GmERF promoters give high wound-inducible expression in transgenic soybean. The 2011 In Vitro Biology Meeting. June 4-8, 2011. Raleigh, NC.

Semsang N, Nemes CA, De la Torre C, Hernandez-Garcia CM, Finer JJ. Isolation and characterization of the GmScream promoter family from soybean. The 2011 In Vitro Biology Meeting. June 4-8, 2011. Raleigh, NC.

De la Torre CM, Hernandez-Garcia CM, Bouchard RA, Finer JJ. Dissection of the strong soybean ubiquitin promoter. The 2011 In Vitro Biology Meeting. June 4-8, 2011. Raleigh, NC.

Hernandez-Garcia CM, Nemes CA, De la Torre C, Rushton PJ, Jones ML, Finer JJ. Discovery of soybean wound-inducible promoters. International Conference on Plant Transformation Technologies II. February 19-22, 2011. Vienna, Austria [Oral Presentation].

Hernandez-Garcia CM, Nemes CA, De la Torre C, Jones ML, Finer JJ. Wound-induced gene expression mediated by novel soybean promoters. 5th Annual Horticulture & Crop Science Graduate Research Retreat. October 15-16, 2010. The Ohio State University. Columbus, OH.

Hernandez-Garcia CM, Bouchard RA, Nemes CA, De la Torre C, Jones ML, Finer JJ. A large set of novel soybean promoters drives exceptionally high levels of constitutive and inducible gene expression in soybean. 13th Biennial Molecular & Cellular Biology of the Soybean Conference 2010. August 8-11, 2010. Durham, NC.

De la Torre CM, Hernandez-Garcia CM, Bouchard RA, Finer JJ. Fine analysis of the soybean Gmubi promoter. 13th Biennial Molecular & Cellular Biology of the Soybean Conference 2010. August 8-11, 2010. Durham, NC.  

Hernandez-Garcia CM, Bouchard RA, Jones ML, Finer JJ. Rapid functional characterization of soybean promoters in transiently- and stably-transformed tissues. The 2010 In Vitro Biology Meeting/12th World Congress of the International Association for Plant Biotechnology (IAPB). June 6-11, 2010. St. Louis, MO.

Lin J, Hernandez-Garcia CM, Bouchard RA, Finer JJ, Redinbaugh MG, Dorrance AE, Qu F. Searching for a RNA silencing suppressor encoded by Bean pod mottle virus in both host and nonhost plants. 2010 OARDC Annual Research Conference. April 22, 2010. Wooster, OH.

Hernandez-Garcia CM, Bouchard RA, Finer JJ. Rapid isolation and analysis of 20 new soybean promoters using two complimentary tools for promoter validation. 4th Annual Horticulture & Crop Science Graduate Research Retreat. October 2-3, 2009. The Ohio State University. Wooster, OH.

Hernandez-Garcia CM, Bouchard RA, Nemes CA, Jones ML, Finer JJ. Rapid isolation and characterization of soybean group IX ERF promoters using two different validation tools. 2009 OARDC Annual Research Conference. April 23, 2009. Columbus, OH.

Hernandez-Garcia CM, Bouchard RA, Nemes CA, Finer JJ. Finer analysis of a strong constitutive soybean promoter in transgenic seedlings and developing plants of soybean. 3rd Annual Horticulture & Crop Science Graduate Research Retreat. October 3-4, 2008. The Ohio State University. Columbus, OH.

Hernandez-Garcia CM, Bouchard RA, Nemes CA, Chiera JM, Finer JJ. Characterization of a soybean (Glycine max) polyubiquitin promoter in transgenic soybean. The 12th Biennial Conference on the Molecular & Cellular Biology of the Soybean. July 20-23, 2008. Indianapolis, IN.

Finer JJ, Chiera JM, Hernandez-Garcia CM, Bouchard RA. Characterization of soybean promoters using GFP expression in transgenic soybean. The 2007 Ohio Plant Biotechnology Consortium Annual Meeting. October 27, 2007. Columbus, OH.

 

Educational Outreach Publications

Hernandez-Garcia CM (2011) Seed Coat Pigmentation in Soybean: An RNA Silencing Mediated Trait. http://www.extension.org/pages/61175  

Hernandez-Garcia CM, Abud G (2011) Using Bioinformatics to Identify Promoters in Genome Sequences. http://www.extension.org/article/32511

Hernandez-Garcia CM, Zhifen Zhang (2011) The Genetics of Pepper Heat: Significant Genes Identified in Capsaicinoid Biosynthesis. http://www.extension.org/article/32603

 

Honors and Awards

Recipient of the highly-competitive 2011 Charles E. Thorne Memorial Associateship from the Ohio Agricultural Research and Development Center (OARDC). This associateship provides wide recognition and financial support for graduate students with outstanding academic achievements.

Second place in poster competition at the 5th Annual Horticulture & Crop Science Graduate Research Retreat. October 15-16, 2010. The Ohio State University. Columbus, OH.

Travel Award and Fellowship Registration to attend 13th Biennial Molecular & Cellular Biology of the Soybean Conference. August 8-11. Durham, NC.

Third place in the graduate student oral presentation competition at the 12th World Congress of the International Association for Plant Biotechnology (IAPB). June 6-11, 2010. St. Louis, MO.

Recipient of the John S. Song Award for 2010 from the Society for In Vitro Biology (SIVB). An award that provides recognition and financial support for students who have contributed and made outstanding achievements in the field of in vitro biology.

Recipient of a 2010 SIVB Student Registration Fellowship to attend the 2010 In Vitro Biology Meeting/12th World Congress of the International Association for Plant Biotechnology (IAPB). June 6-11, 2010. St. Louis, MO.

First place in oral presentation at the 4th Annual Horticulture & Crop Science Graduate Research Retreat. October 2-3, 2009. The Ohio State University. Wooster, OH.

SEEDS: The OARDC Research Enhancement Competitive Grants Program, Graduate Research Competition. Project funded “Cloning and Characterization of Soybean GmERF Promoters”. April 2009- April 2011.

Horticulture and Crop Science Graduate Student Publication Award for 2009 based on the paper “A soybean (Glycine max) polyubiquitin promoter gives strong constitutive expression in transgenic soybean”. This award recognizes the best papers published by a current or recently graduated student in a refereed journal.

Fellowship from Science and Technology Council of Mexico State, given to outstanding graduate-level research projects. 2006

Fellowship from Mexico State to hold a position as a Graduate Student Research Associate at the Genetic Engineering Lab of CIMMYT, given to graduate students with high GPAs. 2005-2006

Fellowship from the Mexican Academy of Science (Academia Mexicana de Ciencias) to pursue a Summer internship in the Postgraduate College in Agriculture Sciences. Summer, 2002

 


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